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Druckenmiller, M. L.; Moon, T. A.; Thoman, R. L. (Ed.)People experience the consequences of a rapidly changing Arctic as the combined effects of physical conditions; responses of biological resources; impacts on infrastructure; decisions influencing adaptive capacities; and both environmental and international influences on economics and well-being. Living and innovating in Arctic environments over millennia, Indigenous Peoples have evolved holistic knowledge providing resilience and sustainability. Indigenous expertise is augmented by scientific abilities to reconstruct past environments and to model and predict future changes. Applying the combined understanding of Indigenous and scientific experts will be important if decision makers (from communities to governments) are to help mitigate and adapt to a rapidly changing Arctic. Considerable discussion among diverse collaborators suggests that addressing unprecedented Arctic environmental changes requires hearing one another, aligning values, and collaborating across knowledge systems, disciplines, and sectors of society.more » « less
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null (Ed.)The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity, and demands for redirection of scientific efforts and criteria to organized research projects. The international Covid19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in Covid19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalogue of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR-chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope labeled form.more » « less
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